Fluorometer
A fluorometer is an analytical instrument designed for fast and accurate measurement of fluorescence properties in samples. Portable fluorometers are especially suitable for on-site field measurements as well as laboratory use
A fluorometer is a sensitive analytical instrument used to measure the fluorescence properties of a sample. Fluorescence occurs when certain substances absorb light at a specific wavelength and then emit light at a longer wavelength. By detecting this emitted light, a fluorometer provides accurate qualitative and quantitative information about the sample.
Portable fluorometers are especially designed for quick field measurements while maintaining laboratory-grade accuracy. These instruments are compact, lightweight, and highly sensitive, allowing them to be used both on-site and in laboratory environments. Their portability makes them ideal for field research, environmental studies, agricultural analysis, and rapid screening applications.
To support outdoor and mobile research, portable fluorometers are engineered with a rugged yet lightweight design, easy handling, and battery-powered operation. This ensures sufficient autonomy for extended fieldwork while maintaining high analytical performance.
Applications of a Portable Fluorometer
- Photosynthetic Activity Studies:Measurement of chlorophyll fluorescence to assess plant health and photosynthetic efficiency.
- Stress Detection in Plants:Identification of environmental, chemical, or biological stress responses.
- Herbicide Activity Monitoring:Evaluation of herbicide effects on plant metabolic processes.
- Mutation and Gene Expression Detection:Detection of fluorescent markers linked to genetic changes.
- Screening and Research Applications:Rapid screening of biological or chemical samples.
- Educational Use:Teaching fluorescence principles in universities and research institutions.
Analysis Modes
- Excitation Mode – Measures fluorescence as excitation wavelength varies
- Emission Mode – Measures emission spectra at a fixed excitation wavelength
- Synchronous Mode – Simultaneously scans excitation and emission wavelengths
Wavelength Ranges
- Excitation Wavelength Range: 250 – 800 nm
- Emission Wavelength Range: 250 – 1000 nm
FLUOROMETER WORKING PRINCIPLE
- The fluorometer operates on the principle of fluorescence emission. When a fluorescent substance absorbs light energy at a specific wavelength, its molecules are excited to a higher energy state. As these molecules return to their ground state, they emit light at a longer wavelength.
- The intensity of this emitted light is directly proportional to the concentration of the fluorescent substance present in the sample. The fluorometer detects and quantifies this emitted light using a sensitive photodetector, enabling precise analysis.
Fluo-100 Highlights
- Simple – User Friendly with 4.3 inch touch screen.
- 2. Easy determination within 3 sec for Protein, DNA & RNA
- 3. High sensitivity Value – Detection limit for DNA is 0.5pg/ul
- 4. Optical channels – Fluorometers are equipped with two fluorescence channels, one for nucleic acid and second for protein quantitation in one detection.
- 5. Five orders of magnitude – Linear Dynamic Range
- 6. Choose the reagent & match it with the instrument.
- 7. Data storage capacity of 1000 samples.
- 8. 0.5ml qPCR tube adapter & 0.2ml qPCR tube adapter
Two detection models of Fluo-100
Application of different fluorescence channel
Conclusion:
- The fluorometer is a powerful, versatile, and highly sensitive analytical instrument designed for fluorescence-based detection in both field and laboratory environments. Its portability, rapid measurement speed, and broad spectral range make it an essential tool for modern biological, agricultural, environmental, and industrial research.
- With robust performance, intuitive operation, and advanced detection capabilities, the fluorometer ensures accurate, repeatable, and reliable results across a wide variety of applications. It is an ideal solution for professionals who require precision fluorescence measurement without compromising mobility or ease of use.